Engineering

57	Ogi, H.; Okamoto, K.; Nagai, H.; Fukunishi, Y.; Hirao, M. (Graduate School of Engineering Science) Replacement-Free Electrodeless Quartz Crystal Microbalance Biosensor Using Nonspecific-Adsorption of Streptavidin on Quartz Analytical Chemistry, 81, 4015-4020 (2009)

58	Ohya, T.; Hayashi, T.; Kiyama, E.; Nishi, H.; Miki, H.; Kobayashi, K.; Honda, K.; Omasa, T.; Ohtake, H. (Graduate School of Engineering) Improved Production of Recombinant Human Antithrombin III in Chinese Hamster Ovary Cells by ATF4 Overexpression Biotechnology and Bioengineering, 100(2), 317-324 (2008) To improve the production of recombinant therapeutic protein in Chinese hamster ovary (CHO) cells, the ATF4 (activating transcription factor 4), which was involved in the mammalian unfolded protein response, was newly cloned from CHO-K1 cells. Overexpression of ATF4 significantly enhanced the recombinant AT-III production. The AT-III concentration in the culture supernatant was twofold greater compared to that observed with parental CHO cells. Our results indicate that overexpression of the ATF4 is a promising means for improving the production of secreted protein pharmaceuticals in CHO cells.

59	Ohtsuka, T.; Streif, S. (Graduate School of Engineering Science) Commutativity of Immersion and Linearization IEEE Transactions on Automatic Control, 54(4), 826-829 (2009) An immersion of a dynamical system is a mapping of the initial state from the original state space to another state space, while exactly preserving the input-output map. In this paper, it is shown that the linearization of the system after immersion has an identical input-output map to the linearization of the original system before immersion. This is potentially useful for applications such as linear control design and sensitivity analysis after nonlinear identification, and has important implications for system approximation by linearization.

60	Ozeki, Y.; Dake, F.; Kajiyama, S.; Fukui, K.; Itoh, K. (Graduate School of Engineering) Analysis and Experimental Assessment of the Sensitivity of Stimulated Raman Scattering Microscopy Optics Express, 17(5), 3651-3658 (2009) This paper theoretically points out that stimulated Raman scattering (SRS) microscopy, which allows label-free biological imaging with high-contrast, can have a sensitivity that is comparable to coherent anti-Stokes Raman scattering (CARS) microscopy, whose sensitivity is sufficient for video-rate operation. Experiments show that the sensitivity of the presented SRS microscope is close to the theoretical limit. These results prove the high-sensitivity nature of the SRS scheme and pave the way for video-rate operation of the SRS microscopy.

61	Sakamoto, M.; Tachikawa, T.; Fujitsuka, M.; Majima, T. (Institute of Scientific and Industrial Research) Two-Laser-Guided Three-Dimensional Microfabrication and Processing in Flexible Polymer Matrix Advanced Materials, 20, 3427-3432 (2008)

62	Sakhaee, E.; Wakamiya, N.; Murata, M. (Graduate School of Information Science and Technology) An Energy-efficient Self-organizing Global Extremity Reporting Scheme for Sensor Networks Wireless Communications and Mobile Computing, 9, 543-555 (2008)

63	Shiraishi, Y.; Miyamoto, R.; Hirai, T. (Research Center for Solar Energy Chemistry) A Hemicyanine-Conjugated Copolymer as a Highly Sensitive Fluorescent Thermometer Langmuir, 24, 4273-4279 (2008) A simple copolymer consisting of N-isopropylacrylamide and hemicyanine units behaves as a fluorescent thermometer. The copolymer dissolved in water shows weak fluorescence at <25 °C, while showing fluorescence enhancement at >25 °C, enabling temperature detection at 25–40 °C. This is driven by a heat-induced phase transition of the polymer from coil to globule state; this allows a transformation of the HC units from the benzenoid form to the fluorescent quinoid form, resulting in fluorescence enhancement. The polymer shows reversible fluorescence response regardless of the heating/cooling process, enabling a simple but accurate temperature detection.

64	Smith, N. I. ^{1, 2} ; Kumamoto, Y. ^2 ; Iwanaga, S. ^2 ; Ando, J. ^2 ; Fujita, K. ^2 ; Kawata, S. ^2 ^1 (Immunology Frontier Research Center) ^2 (Graduate School of Engineering) A Femtosecond Laser Pacemaker for Heart Muscle Cells Optics Express, 16(12), 8604-8616 (2008) We show that controlled exposure to laser light can periodically trigger contractions in muscle cells. Large groups of heart muscle cells synchronized with periodic irradiation by femtosecond laser. The cells undergo a transient increase in intracellular calcium, leading to contraction, following the laser pacemaker. Following irradiation, some cells retained the pacemaking period, while others reverted to original beat frequencies. The mechanisms of these synchronization phenomena will contribute to the general knowledge of heart cell contraction and to understanding the breakdown of normal heart cell function.

65	Sugimoto, Y.; Pou, P.; Custance, O.; Jelinek, P.; Peres, R.; Abe, M.; Morita, S. (Graduate School of Engineering) Complex Patterning by Vertical Interchange Atom Manipulation Using Atomic Force Microscopy Science, 322, 413-417 (2008)

66	Tane, M. ^1 ; Akita, S. ^1 ; Nakano, T. ^2 ; Hagihara, K. ^2 ; Umakoshi, Y. ^2 ; Niinomi, M.; Nakajima, H. ^1 ^1 (Institute of Scientific and Industrial Research) ^2 (Graduate School of Engineering) Peculiar Elastic Behavior of Ti-Nb-Ta-Zr Single Crystals Acta Materialia, 56, 2856-2863 (2008) The cause of a low Young's modulus was investigated in quaternary β-type Ti-Nb-Ta-Zr alloys. It was revealed that a low Young's modulus in a polycrystalline form is caused by the low shear modulus c′, related to the low β-phase stability and low c ₄₄ in the single crystal. Furthermore, it was found that the single crystals had strong orientation dependence on Young's modulus, where the Young's modulus in the 〈1 0 0〉-direction E ₁₀₀ is the lowest of all crystallographic orientations. For quaternary Ti-29Nb-13Ta-4.6Zr alloy (mass%), E ₁₀₀ is only ∼35 GPa, which is similar to Young's modulus of human cortical bones.

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