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Localization of fibronectin-binding
protein, FbaA, on
S. pyogenes
surfaces.
FbaA was visualized as red images
and
S. pyogenes
organisms were stained
as green images. The localization
of FbaA led us speculates that FbaA
functions as an adhesin/invasin and
could be a potential vaccine candidate
for M1 strains of
S. pyogenes
infection.
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Investigation of Fn-binding and
immunogenic domains in FbaA. (A)
Schematic diagram of recombinant
GST-FbaA fusion proteins. (B) The
proteins were electrophoresed in
a 10% SDS-PAGE gel and stained with
Coomassie brilliant blue R-250. (C)
Biacore analysis of binding affinity
between immobilized Fn and GST-truncated
FbaA fusion proteins. (D) Survival
plots of BALB/c mice challenged with
various serotypes of
S. pyogenes
.
Mice were subcutaneously immunized
with GST-truncated FbaA fusion proteins
and then challenged with a lethal
dose of
S. pyogenes
.
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