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Research at OU


  Enhanced Adhesion of Endothelial Cells onto a Polypropylene Hollow Fiber Membrane by Plasma Discharge Treatment and High Inoculum Cell Density  

The effect of plasma discharge treatment of a microporous polypropylene hollow fiber (HF) membrane commonly used for gas exchange in a conventional artificial lung on the adhesion of endothelial cells was investigated with the aim of constructing a hybrid artificial lung bearing endothelial cells on the membrane. The initial adhesion density and growth rate of the cells on the membrane were markedly increased with plasma discharge treatment (13.56 MHz, 30 W) of the membrane for 5 to 20 min in the presence of 0.05 mmHg of various gases such as ammonia, oxygen and water vapor. Treatment of the membrane with ammonia for 5 min resulted in the highest increase in the cell adhesion density on 5 d from 1.4 × 102 to 2.0 × 103 cells/cm2. Increasing the inoculum cell concentration from 3.3 × 105 to 3.2 × 106 cells/ml resulted in the initial cell adhesion of 0.9 × 105 cells/cm2 even after 1 day. It was observed under a microscope that the cells spread well on the membrane and were distributed uniformly to cover almost all of the surface area of the membrane.


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