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Cinet Seminar: "Optical analysis of the neural dynamics in the intact animal ~From synapse to brain regions~"

2014-2-13 (Thu) 1:30 p.m. - 2:30 p.m.

Lecturer: KITAMURA Kazuo, Professor, The University of Tokyo

Neural activities are highly dynamic in response to external stimuli and it depends on the internal state of the brain. To understand the mechanism and physiological relevance of such neural dynamics, it is critical to uncover the spatiotemporal pattern of neural activities from individual synapses to brain regions in the intact brain. Recent advances in optical techniques including two-photon imaging and optogenetics in the intact rodents can facilitate the analysis for the spatiotemporal pattern of the neural dynamics in vivo. I would like to introduce our on-going projects for understanding the sensorimotor information processing in the neocortex and the cerebellum of mouse.

1) Spatiotemporal pattern of synaptic inputs in the somatosensory cortex of mouse: Neurons integrate thousands of synaptic inputs in a highly nonlinear manner and generate distinct patterns of spike output depending on the spatiotemporal input sequences. We visualized individual sensory synaptic inputs to layer 2/3 neurons in the barrel cortex of mouse and found that the spatiotemporal patterns of synaptic inputs were highly heterogeneous. Both spontaneous and sensory-evoked inputs were locally clustered, and vast majority of sensory inputs were directed to a small subset of spines. We also found that sensory inputs share the same spatial pattern as spontaneous events. The results suggest that barrel cortex neurons produce similar pattern of sensory-evoked and spontaneous activities by receiving similar pattern of synaptic inputs.

2) Microzonal organization of the cerebellar cortex during sensorimotor activities in behaving mouse: Longitudinal zonal structure is the hallmark of the functional cerebellar network. This zonal organization of the cerebellar cortex is defined as climbing fiber input that produces complex spike synchrony across populations of cerebellar Purkinje cells oriented in the parasagittal axis. Elucidating the fine spatial structure of this synchrony is crucial for understanding its role in the encoding and processing of sensorimotor information within the olivocerebellar cortical circuit. We performed calcium imaging in awake mice performing a lick/no-lick task, and identified the task-related population activity of Purkinje cells. Further analysis will reveal functional significance of the longitudinal compartments for sensorimotor activities.

Don't miss this chance to listen to an outstanding leader in the field of nanobiology!

Date: 2014-2-13 (Thu) 1:30 p.m. - 2:30 p.m.
Venue: 3rd Floor Seminar Room, Nano-Biology Bldg., Graduate School of Frontier Biosciences
Registration: Not necessary.
Contact: Hayakawa, Administrative Office for Large-Scale Education and Research Projects

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